Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication.

Biochemical and biophysical research communications - 5 May 2018

Aye Seaim Lwin, Fujiwara Kei, Ueki Asuka, Doi Nobuhide

Abstract excerpt

Although compartmentalized self-replication (CSR) and compartmentalized partnered replication (CPR) are powerful tools for directed evolution of proteins and gene circuits, limitations remain in the emulsion PCR process with the wild-type Taq DNA polymerase used so far, including long run times, low amounts of product, and false negative results due to inhibitors. In this study, we developed a high-efficiency...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Amino Acid Sequence
Share to Directed Molecular Evolution

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to DNA Polymerase I

Share to DNA Replication

Share to Mutation

Share to Protein Engineering

Share to Thermus thermophilus

Source

Identifiers

PMID: 29550479
DOI: 10.1016/j.bbrc.2018.03.098

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Engineering of DNA polymerase I from Thermus thermophilus using compartmentalized self-replication.PMID 29550479Read ↗

Directed evolution of DNA polymerase, RNA polymerase and reverse transcriptase activity in a single polypeptide.Semantic proximity Read ↗

Compartmentalized partnered replication for the directed evolution of genetic parts and circuits.Semantic proximity Read ↗

In vitro evolution of phi29 DNA polymerase using isothermal compartmentalized self replication technique.Semantic proximity Read ↗

Directed evolution of polymerase function by compartmentalized self-replication.Semantic proximity Read ↗

Engineering of T7 DNA-dependent RNA polymerase with activity at elevated temperature.Semantic proximity Read ↗

Directed co-evolution of interacting protein-peptide pairs by compartmentalized two-hybrid replication (C2HR).Semantic proximity Read ↗

Directed polymerase evolution.Semantic proximity Read ↗

An evolved, orthogonal ssDNA generator for targeted hypermutation of multiple genomic loci.Semantic proximity Read ↗

In vitro evolution of phi29 DNA polymerases through compartmentalized gene expression and rolling-circle replication.Semantic proximity Read ↗

Mutations to create thermostable reverse transcriptase with bacterial family A DNA polymerase from Thermotoga petrophila K4.Semantic proximity Read ↗

Engineered DNA polymerases in biotechnology.Semantic proximity Read ↗

Improved PCR performance using mutant Tpa-S DNA polymerases from the hyperthermophilic archaeon Thermococcus pacificus.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.