Article
PRICE: direct and robust detection of microRNAs at single-nucleotide resolution.
Nature communications - 12 Feb 2026
Wang Buhua, Zhou Shuai, Zhang Xi, Wang Rui, Huang Shuo, Li Anyi, He Haowei, Zhang Yingai, Wei Yangdao, Yang Zhiqing, Song Fengge, Li Xiangpeng, Wan Xinyi, Shen Yi, Ma Chunxin, Mao Haimei, Ledesma-Amaro Rodrigo, Yin Dapeng, Wei Qingshan, Deng Ruijie, Yang Tao, Liu Shanrong, Wan Yi, Mao Chuanbin
Abstract excerpt
Accurate single-nucleotide discrimination of miRNA is clinically vital because small sequence variations can have significant phenotypic and clinical consequences, yet existing techniques can only detect single nucleotide variations (SNVs) at specific loci. Here, we present a generalized peptide nucleic acid (PNA) mediated CRISPR/Cas13a system (PRICE), enabling detection of SNVs in miRNA sequence without...
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