One-Pot Endonucleolytically Exponentiated Rolling Circle Amplification by CRISPR-Cas12a Affords Sensitive, Expedited Isothermal Detection of MicroRNAs

Abstract excerpt

MicroRNAs (miRNAs) are a class of short non-coding RNAs that play essential roles in gene expression regulation. While miRNAs offer a promising source for developing potent cancer biomarkers, the progress towards clinical utilities remains largely limited, due in part to the long-standing challenge in sensitive, specific, and robust detection of miRNAs in human biofluids. Emerging next-generation molecular technol...

Topics

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• Clustered Regularly Interspaced Short Palindromic Repeats
• CRISPR-Cas Systems
• Genotyping Techniques
• Leukemia, Myeloid, Acute
• Nucleic Acid Amplification Techniques
• Pancreatic Neoplasms
• Point-of-Care Systems
• Polymorphism, Single Nucleotide

Identifiers and source

Literature Corpus work

7cedf283-b3d6-5d31-a7e9-f9ba4b4b6403

DOI

10.1101/2022.05.01.490215