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A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNA

2024-01-23

Abstract excerpt

<title>Abstract</title> <p>The complete 40-nt CRISPR RNA (crRNA) of Cas12a can be artificially divided into two parts, including the 20-nt scaffold RNA with fixed sequences and the 20-nt spacer RNA with variable sequences, respectively. Herein, we found that Cas12a protein, scaffold RNA, and spacer RNA can be reassembled into an active ribonucleoprotein (RNP) which has a <italic>trans</italic>-cleavage activity c...

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Literature Corpus work
9cb781ad-f484-58cb-9fae-966f4589cc44
DOI
10.21203/rs.3.rs-3858975/v1
Open publication

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A split crRNA with CRISPR-Cas12a enables highly sensitive, selective, and multiplexed detection of RNA and DNADOI 10.21203/rs.3.rs-3858975/v1
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