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Thermophilic Chassis-Enabled High-Throughput Selection of a Thermostable Fluorogenic Reporter.

ACS synthetic biology - 17 Oct 2025

Shin Sang-Min, Triola Ellin-Kristina H, Granja-Travez Rommel S, López Cesar A, Wood Neely M, Riley Lauren A, Guss Adam M, Dale Taraka, Jha Ramesh K

Abstract excerpt

Thermostable proteins show increased shelf life and performance at elevated temperatures and under harsh conditions, resulting in lower costs for various industrial and biotechnological applications. However, due to a limited understanding of the relationship between stability and function, protein stabilization remains primarily a trial-and-error approach. Therefore, building a combinatorial library of mutations...

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PMID: 41058484
DOI: 10.1021/acssynbio.5c00573

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