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Article

A dual-reporter system for investigating and optimizing protein translation and folding in E. coli.

Nature communications - 19 Oct 2021

Zutz Ariane, Hamborg Louise, Pedersen Lasse Ebdrup, Kassem Maher M, Papaleo Elena, Koza Anna, Herrgård Markus J, Jensen Sheila Ingemann, Teilum Kaare, Lindorff-Larsen Kresten, Nielsen Alex Toftgaard

Abstract excerpt

Strategies for investigating and optimizing the expression and folding of proteins for biotechnological and pharmaceutical purposes are in high demand. Here, we describe a dual-reporter biosensor system that simultaneously assesses in vivo protein translation and protein folding, thereby enabling rapid screening of mutant libraries. We have validated the dual-reporter system on five different proteins and find an...

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Identifiers

PMID: 34667164
DOI: 10.1038/s41467-021-26337-1
PMCID: PMC8526717

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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