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Article

A Computational Library Design Protocol for Rapid Improvement of Protein Stability: FRESCO.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Wijma Hein J, Fürst Maximilian J L J, Janssen Dick B

Abstract excerpt

The ability to stabilize enzymes and other proteins has wide-ranging applications. Most protocols for enhancing enzyme stability require multiple rounds of high-throughput screening of mutant libraries and provide only modest improvements of stability. Here, we describe a computational library design protocol that can increase enzyme stability by 20-35 °C with little experimental screening, typically fewer than...

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Identifiers

PMID: 29086304
DOI: 10.1007/978-1-4939-7366-8_5

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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