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Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Mita Marie, Wongso Devina, Ueda Hiroshi, Tsuboi Takashi, Kitaguchi Tetsuya

Abstract excerpt

Advances in live-cell imaging have been accelerated by the development of various fluorescent indicators. However, indicators that are suitable for multicolor imaging remain a challenge to develop. Herein, we have developed a single fluorescent protein (FP)-based indicator using a semirational molecular design and a molecular evolution approach. We first inserted a ligand-binding domain into the vicinity of an FP...

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PMID: 34050465
DOI: 10.1007/978-1-0716-1258-3_9

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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