Article
Development of a Single Fluorescent Protein-Based Green Glucose Indicator by Semirational Molecular Design and Molecular Evolution.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Mita Marie, Wongso Devina, Ueda Hiroshi, Tsuboi Takashi, Kitaguchi Tetsuya
Abstract excerpt
Advances in live-cell imaging have been accelerated by the development of various fluorescent indicators. However, indicators that are suitable for multicolor imaging remain a challenge to develop. Herein, we have developed a single fluorescent protein (FP)-based indicator using a semirational molecular design and a molecular evolution approach. We first inserted a ligand-binding domain into the vicinity of an FP...
Topics
- Evolution, Molecular
- Fluorescent Dyes
- Glucose
- Green Fluorescent Proteins
- HeLa Cells
- Humans
- Molecular Imaging
- Mutagenesis, Site-Directed
- Mutation
- Polymerase Chain Reaction