Rapid, randomized development of genetically encoded FRET sensors for small molecules.

Abstract excerpt

Imaging small molecules in living cells can be achieved with genetically encoded fluorescent reporters. The most common sensors emit a signal generated by Förster resonance energy transfer (FRET), which occurs when two fluorescent proteins that flank a sensor protein are moved into close proximity. This movement occurs when the sensor protein binds a ligand of interest. The structural requirements for creating...

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