Article
Rapid, randomized development of genetically encoded FRET sensors for small molecules.
The Analyst - 7 Jul 2015
Peroza Estevão Aun, Boumezbeur Ahmed-Hocine, Zamboni Nicola
Abstract excerpt
Imaging small molecules in living cells can be achieved with genetically encoded fluorescent reporters. The most common sensors emit a signal generated by Förster resonance energy transfer (FRET), which occurs when two fluorescent proteins that flank a sensor protein are moved into close proximity. This movement occurs when the sensor protein binds a ligand of interest. The structural requirements for creating...
Topics
- Biosensing Techniques
- Fluorescence Resonance Energy Transfer
- Glucose
- Ligands
- Mutation
- Proteins
- Pyruvic Acid
- Thiamine
- Time Factors