Article
Rapid Thermostabilization of Bacillus thuringiensis Serovar Konkukian 97-27 Dehydroshikimate Dehydratase through a Structure-Based Enzyme Design and Whole Cell Activity Assay.
ACS synthetic biology - 20 Jan 2017
Harrington Lucas B, Jha Ramesh K, Kern Theresa L, Schmidt Emily N, Canales Gustavo M, Finney Kellan B, Koppisch Andrew T, Strauss Charlie E M, Fox David T
Abstract excerpt
Thermostabilization of an enzyme with complete retention of catalytic efficiency was demonstrated on recombinant 3-dehydroshikimate dehydratase (DHSase or wtAsbF) from Bacillus thuringiensis serovar konkukian 97-27 (hereafter, B. thuringiensis 97-27). The wtAsbF is relatively unstable at 37 °C, in vitro (t1/237 = 15 min), in the absence of divalent metal. We adopted a structure-based design to identify...
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