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Article

Experimental Protocols for Generating Focused Mutant Libraries and Screening for Thermostable Proteins.

Methods in enzymology - 1 Jan 2018

Fürst Max J L J, Martin Caterina, Lončar Nikola, Fraaije Marco W

Abstract excerpt

Many proteins are rapidly deactivated when exposed to high or even ambient temperatures. This cannot only impede the study of a particular protein, but also is one of the major reasons why enzyme catalysis is still widely unable to compete with established chemical processes. Furthermore, differences in protein stability are a challenge in synthetic biology, when individual modules prove to be incompatible. The...

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PMID: 30173762
DOI: 10.1016/bs.mie.2018.04.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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