Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

GGAssembler: Precise and economical design and synthesis of combinatorial mutation libraries.

Protein science : a publication of the Protein Society - 1 Oct 2024

Hoch Shlomo Yakir, Netzer Ravit, Weinstein Jonathan Yaacov, Krauss Lucas, Hakeny Karen, Fleishman Sarel Jacob

Abstract excerpt

Golden Gate assembly (GGA) can seamlessly generate full-length genes from DNA fragments. In principle, GGA could be used to design combinatorial mutation libraries for protein engineering, but creating accurate, complex, and cost-effective libraries has been challenging. We present GGAssembler, a graph-theoretical method for economical design of DNA fragments that assemble a combinatorial library that encodes any...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA
Share to Gene Library

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to Peptide Library

Share to Protein Engineering

Source

Open in PubMed Open full text

Identifiers

PMID: 39283039
DOI: 10.1002/pro.5169
PMCID: PMC11403590

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 4 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

GGAssembler: Precise and economical design and synthesis of combinatorial mutation libraries.PMID 39283039Read ↗

Mutation Maker, An Open Source Oligo Design Platform for Protein Engineering.Semantic proximity Read ↗

Fast and efficient template-mediated synthesis of genetic variants.Semantic proximity Read ↗

Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.Semantic proximity Read ↗

An end-to-end automated platform process for high-throughput engineering of next-generation multi-specific antibody therapeuticsSemantic proximity Read ↗

Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis.Semantic proximity Read ↗

Optimization of multi-site nicking mutagenesis for generation of large, user-defined combinatorial libraries.Semantic proximity Read ↗

Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries.Semantic proximity Read ↗

Polishing the craft of genetic diversity creation in directed evolution.Semantic proximity Read ↗

Improving genotyped functional screening: A versatile closed-tube PCR for Illumina library generation reduces background sequences in multiplexed sequencing.Semantic proximity Read ↗

Determination of a Screening Metric for High Diversity DNA Libraries.Semantic proximity Read ↗

Facile generation of antibody heavy and light chain diversities for yeast surface display by Golden Gate CloningSemantic proximity Read ↗

The evolving art of creating genetic diversity: From directed evolution to synthetic biology.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.