Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Fast and efficient template-mediated synthesis of genetic variants.

Nature methods - 1 Jun 2023

Liu Liyuan, Huang Yiming, Wang Harris H

Abstract excerpt

Efficient methods for the generation of specific mutations enable the study of functional variations in natural populations and lead to advances in genetic engineering applications. Here, we present a new approach, mutagenesis by template-guided amplicon assembly (MEGAA), for the rapid construction of kilobase-sized DNA variants. With this method, many mutations can be generated at a time to a DNA template at...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to COVID-19
Share to DNA
Share to Escherichia coli

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Mutation

Share to RNA, Viral

Share to SARS-CoV-2

Source

Open in PubMed Open full text

Identifiers

PMID: 37127666
DOI: 10.1038/s41592-023-01868-1
PMCID: PMC12066172

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Fast and efficient template-mediated synthesis of genetic variants.PMID 37127666Read ↗

A versatile, simple, and rapid (VSR) method for generating gene clones, diverse mutants, and short gene fragments for molecular biology applications.Semantic proximity Read ↗

A Rapid, High-Throughput Method for the Construction of Mutagenesis Libraries.Semantic proximity Read ↗

Genome-wide mapping of mutations at single-nucleotide resolution for protein, metabolic and genome engineering.Semantic proximity Read ↗

High-throughput functional variant screens via in vivo production of single-stranded DNA.Semantic proximity Read ↗

MOSAIC: A Highly Efficient, One-Step Recombineering Approach to Plasmid Editing and Diversification.Semantic proximity Read ↗

A cost-effective and scalable barcoded library construction method for deep mutational scanning studies.Semantic proximity Read ↗

Saturation Mutagenesis Genome Engineering of Infective ΦX174 Bacteriophage via Unamplified Oligo Pools and Golden Gate Assembly.Semantic proximity Read ↗

Drawing a high-resolution functional map of adeno-associated virus capsid by massively parallel sequencing.Semantic proximity Read ↗

RLB-MALBAC: A rapid, non-transposase method for synthesizing unbiased sequencing libraries from low amount of genomic DNA for Nanopore sequencingSemantic proximity Read ↗

GGAssembler: Precise and economical design and synthesis of combinatorial mutation libraries.Semantic proximity Read ↗

Single-base sequencing for rapid screening of plasmids for inserts with known mutations and correct orientation.Semantic proximity Read ↗

Directed evolution of DNA polymerases for next-generation sequencing.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.