Article
Facile generation of antibody heavy and light chain diversities for yeast surface display by Golden Gate Cloning
22 Nov 2018
Abstract excerpt
Antibodies can be successfully engineered and isolated by yeast or phage display of combinatorial libraries. Still, generation of libraries comprising heavy chain as well as light chain diversities is a cumbersome process involving multiple steps. Within this study, we set out to compare the output of yeast display screening of antibody Fab libraries from immunized rodents that were generated by Golden Gate...
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