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Improving genotyped functional screening: A versatile closed-tube PCR for Illumina library generation reduces background sequences in multiplexed sequencing.

SLAS technology - 1 Jul 2026

Oksanen Sami, Huovinen Tuomas, Lamminmäki Urpo

Abstract excerpt

We present an improved version of DNA indexing platform allowing geno-phenotype analysis of all binder protein clones arrayed for high-throughput screening. The method was optimized for two primer pairs with differing annealing temperatures (>10 °C), resulting in plate and well ID barcoding in a single, closed-tube PCR. As compared to our earlier hierarchical indexing, the closed-tube approach enhanced the...

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PMID: 42144075
DOI: 10.1016/j.slast.2026.100429

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