Article
Facile Assembly of Combinatorial Mutagenesis Libraries Using Nicking Mutagenesis.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2022
Kirby Monica B, Whitehead Timothy A
Abstract excerpt
Combinatorial mutagenesis is a method where multiple user-defined mutations are encoded at defined positions in a sequence. Combinatorial mutagenic libraries can be used in a variety of applications including evaluating fundamental questions about molecular evolution, directed evolution workflows for enzyme engineering, and in better understanding of biological processes like antibody affinity maturation. Here,...
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