Article
Optimization of multi-site nicking mutagenesis for generation of large, user-defined combinatorial libraries.
Protein engineering, design & selection : PEDS - 15 Feb 2021
Kirby Monica B, Medina-Cucurella Angélica V, Baumer Zachary T, Whitehead Timothy A
Abstract excerpt
Generating combinatorial libraries of specific sets of mutations are essential for addressing protein engineering questions involving contingency in molecular evolution, epistatic relationships between mutations, as well as functional antibody and enzyme engineering. Here we present optimization of a combinatorial mutagenesis method involving template-based nicking mutagenesis, which allows for the generation of...
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