Article
Split & mix assembly of DNA libraries for ultrahigh throughput on-bead screening of functional proteins.
Nucleic acids research - 19 Jun 2020
Lindenburg Laurens, Huovinen Tuomas, van de Wiel Kayleigh, Herger Michael, Snaith Michael R, Hollfelder Florian
Abstract excerpt
Site-saturation libraries reduce protein screening effort in directed evolution campaigns by focusing on a limited number of rationally chosen residues. However, uneven library synthesis efficiency leads to amino acid bias, remedied at high cost by expensive custom synthesis of oligonucleotides, or through use of proprietary library synthesis platforms. To address these shortcomings, we have devised a method...
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