Article
Generic Reporter Sets for Colorimetric Multiplex dPCR Demonstrated with 6-Plex SNP Quantification Panels.
International journal of molecular sciences - 17 Aug 2024
Neugebauer Maximilian, Calabrese Silvia, Müller Sarah, Truong Truong-Tu, Juelg Peter, Borst Nadine, Hutzenlaub Tobias, Dazert Eva, Bubnoff Nikolas Christian Cornelius von, von Stetten Felix, Lehnert Michael
Abstract excerpt
Digital PCR (dPCR) is a powerful method for highly sensitive and precise quantification of nucleic acids. However, designing and optimizing new multiplex dPCR assays using target sequence specific probes remains cumbersome, since fluorescent signals must be optimized for every new target panel. As a solution, we established a generic fluorogenic 6-plex reporter set, based on mediator probe technology, that...
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