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Article

Multiplex Digital PCR Assay to Detect Multiple KRAS and GNAS Mutations Associated with Pancreatic Carcinogenesis from Minimal Specimen Amounts.

The Journal of molecular diagnostics : JMD - 1 Jun 2023

Maeda Chiho, Ono Yusuke, Hayashi Akihiro, Takahashi Kenji, Taniue Kenzui, Kakisaka Rika, Mori Miyuki, Ishii Takahiro, Sato Hiroki, Okada Tetsuhiro, Kawabata Hidemasa, Goto Takuma, Tamamura Nobue, Omori Yuko, Takahashi Kuniyuki, Katanuma Akio, Karasaki Hidenori, Liss Andrew Scott, Mizukami Yusuke

Abstract excerpt

Digital PCR (dPCR) allows for highly sensitive quantification of low-frequency mutations and facilitates early detection of cancer. However, low-throughput targeting of single hotspots in dPCR hinders variant specification when multiple probes are used. We developed a dPCR method to simultaneously identify major variants related to pancreatic carcinogenesis. Using a two-dimensional plot of droplet fluorescence...

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Identifiers

PMID: 36965665
DOI: 10.1016/j.jmoldx.2023.02.007

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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