Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

10-Plex Digital Polymerase Chain Reaction with Four-Color Melting Curve Analysis for Simultaneous KRAS and BRAF Genotyping.

Analytical chemistry - 1 Sept 2020

Nakagawa Tatsuo, Tanaka Junko, Harada Kunio, Shiratori Akiko, Shimazaki Yuzuru, Yokoi Takahide, Uematsu Chihiro, Kohara Yoshinobu

Abstract excerpt

Digital PCR (dPCR) is a promising method for performing liquid biopsies that quantifies nucleic acids more sensitively than real-time PCR. However, dPCR shows large fluctuations in the fluorescence intensity of droplets or wells due to insufficient PCR amplification in the small partitions, limiting the multiplexing capability of using the fluorescence intensity. In this study, we propose a measurement method...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Fluorescence
Share to Genotype

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Liquid Biopsy

Share to Molecular Probes

Share to Proto-Oncogene Proteins B-raf

Share to Proto-Oncogene Proteins p21(ras)

Share to Real-Time Polymerase Chain Reaction

Source

Identifiers

PMID: 32786457
DOI: 10.1021/acs.analchem.0c01704

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

10-Plex Digital Polymerase Chain Reaction with Four-Color Melting Curve Analysis for Simultaneous KRAS and BRAF Genotyping.PMID 32786457Read ↗

Efficient and accurate KRAS genotyping using digital PCR combined with melting curve analysis for ctDNA from pancreatic cancer patients.Semantic proximity Read ↗

Multiplex Digital PCR Assay to Detect Multiple KRAS and GNAS Mutations Associated with Pancreatic Carcinogenesis from Minimal Specimen Amounts.Semantic proximity Read ↗

Six-Color Multiplex Digital PCR Assays for Comprehensive Screening and Identification of Multiple Driver Mutations Associated with Pancreatic Carcinogenesis.Semantic proximity Read ↗

A Novel Multiplex Droplet Digital PCR Assay to Identify and Quantify KRAS Mutations in Clinical SpecimensSemantic proximity Read ↗

Enhanced ratio of signals enables digital mutation scanning for rare allele detection.Semantic proximity Read ↗

A Multiplex Assay for Fast PIK3CA Hotspot Mutation Characterization in a Single Specimen by 3-Color Digital PCR Analysis.Semantic proximity Read ↗

Competitive amplification of differentially melting amplicons (CADMA) enables sensitive and direct detection of all mutation types by high-resolution melting analysis.Semantic proximity Read ↗

All-In-One OsciDrop Digital PCR System for Automated and Highly Multiplexed Molecular Diagnostics.Semantic proximity Read ↗

Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.Semantic proximity Read ↗

Combination of fluorescence color and melting temperature as a two-dimensional label for homogeneous multiplex PCR detection.Semantic proximity Read ↗

Single-Color Digital PCR Provides High-Performance Detection of Cancer Mutations from Circulating DNA.Semantic proximity Read ↗

Reporter emission multiplexing in digital PCRs (REM-dPCRs): direct quantification of multiple target sequences per detection channel by population specific reporters.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.