Article
Reporter emission multiplexing in digital PCRs (REM-dPCRs): direct quantification of multiple target sequences per detection channel by population specific reporters.
The Analyst - 5 Oct 2023
Calabrese Silvia, Markl Anja M, Neugebauer Maximilian, Krauth Stefanie J, Borst Nadine, von Stetten Felix, Lehnert Michael
Abstract excerpt
Digital PCRs (dPCRs) are widely used methods for the detection and quantification of rare abundant sequences relevant to fields such as liquid biopsy or oncology. In order to increase the information content and save valuable sample materials, there is a significant need for digital multiplexing methods that are easy to establish, analyse, and interpret, and ideally allow the usage of existing lab equipment....
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