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Novel Multiplexing Strategies for Quantification of Rare Alleles Using ddPCR.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Alcaide Miguel, Morin Ryan D

Abstract excerpt

Droplet digital PCR (ddPCR) has come to be regarded as the gold standard for the ultrasensitive detection and absolute quantification of closely related DNA sequences within complex mixtures. Most ddPCR assays to date, however, rely on sets of hydrolysis probes conjugated with dyes having different emission spectra to allow independent counting of rare mutant and wild-type alleles. Here, we describe a set of...

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PMID: 29717449
DOI: 10.1007/978-1-4939-7778-9_16

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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