Article
Multiplex digital PCR: breaking the one target per color barrier of quantitative PCR.
Lab on a chip - 7 Jul 2011
Zhong Qun, Bhattacharya Smiti, Kotsopoulos Steven, Olson Jeff, Taly Valérie, Griffiths Andrew D, Link Darren R, Larson Jonathan W
Abstract excerpt
Quantitative polymerase chain reactions (qPCR) based on real-time PCR constitute a powerful and sensitive method for the analysis of nucleic acids. However, in qPCR, the ability to multiplex targets using differently colored fluorescent probes is typically limited to 4-fold by the spectral overlap of the fluorophores. Furthermore, multiplexing qPCR assays requires expensive instrumentation and most often lengthy...
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