Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2017

Pekin Deniz, Taly Valerie

Abstract excerpt

We describe a droplet microfluidics method to screen for multiple mutations of a same oncogene in a single experiment using passive droplet fusion. Genomic DNA from H1573 cell-line was screened for the presence of the six common mutations of the KRAS oncogene as well as wild-type sequences with a detection efficiency of 98 %. Furthermore, the mutant allelic fraction of the cell-line was also assessed correctly...

Topics

Join the communities discussing this publication.

Multiplex Polymerase Chain Reaction
Biomarkers, Tumor
Genes, ras
Microfluidic Analytical Techniques
Microfluidics

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Real-Time Polymerase Chain Reaction

Statistics as Topic

Source

Identifiers

PMID: 28044293
DOI: 10.1007/978-1-4939-6734-6_11

Genes

KRAS

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 1 Sept 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

0

discussions

0

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Multiplex Detection of KRAS Mutations Using Passive Droplet Fusion.PMID 28044293Read ↗

Droplet-Based Microfluidics Digital PCR for the Detection of KRAS Mutations.Semantic proximity Read ↗

Quantitative and sensitive detection of rare mutations using droplet-based microfluidics.Semantic proximity Read ↗

Multiple single cell screening and DNA MDA amplification chip for oncogenic mutation profiling.Semantic proximity Read ↗

A Novel Multiplex Droplet Digital PCR Assay to Identify and Quantify KRAS Mutations in Clinical SpecimensSemantic proximity Read ↗

Reporter emission multiplexing in digital PCRs (REM-dPCRs): direct quantification of multiple target sequences per detection channel by population specific reporters.Semantic proximity Read ↗

Multiplex Enrichment and Detection of Rare KRAS Mutations in Liquid Biopsy Samples using Digital Droplet Pre-AmplificationSemantic proximity Read ↗

Multiplex Digital PCR Assay to Detect Multiple KRAS and GNAS Mutations Associated with Pancreatic Carcinogenesis from Minimal Specimen Amounts.Semantic proximity Read ↗

Single-Cell Digital Microfluidic Mass Spectrometry Platform for Efficient and Multiplex Genotyping of Circulating Tumor Cells.Semantic proximity Read ↗

Multiplex Enrichment and Detection of Rare KRAS Mutations in Liquid Biopsy Samples using Digital Droplet Pre-AmplificationSemantic proximity Read ↗

Multiplex Digital Polymerase Chain Reaction on a Droplet Array SlipChip for Analysis of KRAS Mutations in Pancreatic Cancer.Semantic proximity Read ↗

Monitoring of Response and Resistance in Plasma of EGFR-Mutant Lung Cancer Using Droplet Digital PCR.Semantic proximity Read ↗

Non-invasive profiling of KRAS mutations in lung cancer using droplet digital polymerase chain reaction.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.