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Highly Sensitive and Multiplexed Detection of Low-Frequency Mutation in Fragmented ctDNA by a Dual-Role Mediator Blocker Amplification Strategy.

Analytical chemistry - 14 Oct 2025

Zhang Rui, Pu Zhening, Xu Daxing, Yin Ying, Wei Bingbing, Wang Lei, Liu Zhaocheng

Abstract excerpt

The detection of mutations in circulating tumor DNA (ctDNA) is challenging due to the significant fragmentation of ctDNA and the high prevalence of the wild-type template. Additionally, variant detection through qPCR is typically dependent on target-specific fluorescence probes, and no more than five targets can be identified in a single reaction due to the limited fluorescence colors in thermal cyclers. To...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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PMID: 41042200
DOI: 10.1021/acs.analchem.5c04894

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