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Directed Evolution of Escherichia coli Nissle 1917 to Utilize Allulose as Sole Carbon Source.

Small methods - 1 Aug 2024

Xu Bo, Liu Li-Hua, Lai Shijing, Chen Jingjing, Wu Song, Lei Wei, Lin Houliang, Zhang Yu, Hu Yucheng, He Jingtao, Chen Xipeng, He Qian, Yang Min, Wang Haimei, Zhao Xuemei, Wang Man, Luo Haodong, Ge Qijun, Gao Huamei, Xia Jiaqi, Cao Zhen, Zhang Baoxun, Jiang Ao, Wu Yi-Rui

Abstract excerpt

Sugar substitutes are popular due to their akin taste and low calories. However, excessive use of aspartame and erythritol can have varying effects. While D-allulose is presently deemed a secure alternative to sugar, its excessive consumption is not devoid of cellular stress implications. In this study, the evolution of Escherichia coli Nissle 1917 (EcN) is directed to utilize allulose as sole carbon source...

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PMID: 38415955
DOI: 10.1002/smtd.202301385

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