Enhancing Stability and Catalytic Activity of d-Allulose 3-Epimerase through Multistrategy Computational Design and Cross-Regional Advantageous Mutations.

Abstract excerpt

d-Allulose 3-epimerase (DAEase) derived from Clostridium bolteae has excellent properties in the catalytic production of d-allulose, a rare sugar with unique biological functions. However, the industrial application of C. bolteae DAEase (Cb-DAEase) for d-allulose production is hindered by its low enzyme activity, poor long-term thermostability, and pH tolerance. In this study, we identified potential noncatalytic...

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• Biocatalysis
• Carbohydrate Epimerases
• Clostridium
• Enzyme Stability
• Fructose