Cooperativity between Cas9 and hyperactive AID establishes broad and diversifying mutational footprints in base editors.

Abstract excerpt

The partnership of DNA deaminase enzymes with CRISPR-Cas nucleases is now a well-established method to enable targeted genomic base editing. However, an understanding of how Cas9 and DNA deaminases collaborate to shape base editor (BE) outcomes has been lacking. Here, we support a novel mechanistic model of base editing by deriving a range of hyperactive activation-induced deaminase (AID) base editors (hBEs) and...

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• Share to AICDA (Activation-Induced Cytidine Deaminase)
• Share to CRISPR-Associated Protein 9