Article
Programmable base editing of A•T to G•C in genomic DNA without DNA cleavage.
Nature - 23 Nov 2017
Gaudelli Nicole M, Komor Alexis C, Rees Holly A, Packer Michael S, Badran Ahmed H, Bryson David I, Liu David R
Abstract excerpt
The spontaneous deamination of cytosine is a major source of transitions from C•G to T•A base pairs, which account for half of known pathogenic point mutations in humans. The ability to efficiently convert targeted A•T base pairs to G•C could therefore advance the study and treatment of genetic diseases. The deamination of adenine yields inosine, which is treated as guanine by polymerases, but no enzymes are...
Topics
- Adenosine Deaminase
- Base Pairing
- CRISPR-Associated Proteins
- Cell Line, Tumor
- DNA
- DNA Cleavage
- Gene Editing
- Genome, Human
- HEK293 Cells
- Humans
- Models, Molecular