Article

Highly precise base editing with CC context-specificity using engineered human APOBEC3G-nCas9 fusions

2019-06-03

Abstract excerpt

Cytidine base editors, composed of a cytidine deaminase fused to Cas9 nickase, enable efficient C-to-T conversion in various organisms. However, current base editors can induce unwanted bystander C-to-T conversions when more than one C is present in the activity window of cytidine deaminase, which negatively affects the precision. Here, we develop a new base editor with CC context-specificity using rationally engi...

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Identifiers and source

Literature Corpus work: 810414d9-5ceb-5d2c-8537-12c2e5dd186c
DOI: 10.1101/658351

Open publication

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