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Article

High-precision cytosine base editors by evolving nucleic-acid-recognition hotspots in deaminase.

Nature biotechnology - 1 Apr 2026

Wu Yuan, Xiao Yu-Lan, Tang Weixin

Abstract excerpt

Base editors (BEs), covalent fusions of a cytosine or adenine deaminase with a nuclease-impaired CRISPR protein, mediate site-specific conversion of C:G to T:A (CBEs) or A:T to G:C (ABEs) in the genome. Existing BEs modify all cytosines or adenines within the editing window, which limits their precision. Here we engineer nucleotide and context specificity of the Escherichia coli transfer RNA-specific adenosine...

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Identifiers

PMID: 40624252
DOI: 10.1038/s41587-025-02678-w
PMCID: PMC12240478

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