Article
A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.
Nature biotechnology - 1 Jul 2020
Grünewald Julian, Zhou Ronghao, Lareau Caleb A, Garcia Sara P, Iyer Sowmya, Miller Bret R, Langner Lukas M, Hsu Jonathan Y, Aryee Martin J, Joung J Keith
Abstract excerpt
Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening...
Topics
- Adenine
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Cytosine
- Cytosine Deaminase
- Gene Editing
- HEK293 Cells
- Humans
- Mutation
- RNA