CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions.

doi:10.1158/2159-8290.CD-16-0178

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Article

CRISPR Screens Provide a Comprehensive Assessment of Cancer Vulnerabilities but Generate False-Positive Hits for Highly Amplified Genomic Regions.

Cancer discovery - 1 Aug 2016

Munoz Diana M, Cassiani Pamela J, Li Li, Billy Eric, Korn Joshua M, Jones Michael D, Golji Javad, Ruddy David A, Yu Kristine, McAllister Gregory, DeWeck Antoine, Abramowski Dorothee, Wan Jessica, Shirley Matthew D, Neshat Sarah Y, Rakiec Daniel, de Beaumont Rosalie, Weber Odile, Kauffmann Audrey, McDonald E Robert, Keen Nicholas, Hofmann Francesco, Sellers William R, Schmelzle Tobias, Stegmeier Frank, Schlabach Michael R

Abstract excerpt

UNLABELLED: CRISPR/Cas9 has emerged as a powerful new tool to systematically probe gene function. We compared the performance of CRISPR to RNAi-based loss-of-function screens for the identification of cancer dependencies across multiple cancer cell lines. CRISPR dropout screens consistently identified more lethal genes than RNAi, implying that the identification of many cellular dependencies may require full gene...

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PMID: 27260157
DOI: 10.1158/2159-8290.CD-16-0178

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

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