Article

Massively parallel kinetic profiling of natural and engineered CRISPR nucleases

2019-07-09

Abstract excerpt

Engineered Streptococcus pyogenes ( Sp ) Cas9s and Acidaminococcus sp . ( As ) Cas12a (formerly Cpf1) improve cleavage specificity in human cells. However, the fidelity, enzymatic mechanisms, and cleavage products of emerging CRISPR nucleases have not been profiled systematically across partially mispaired off-target DNA sequences. Here, we describe NucleaSeq— nuclea se digestion and deep seq uencing—a massi...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 3e8f5432-8e4a-51a4-bd93-9bd1dcb0231e
DOI: 10.1101/696393

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

Massively parallel kinetic profiling of natural and engineered CRISPR nucleasesDOI 10.1101/696393Read

Systematic in vitro profiling of off-target affinity, cleavage and efficiency for CRISPR enzymes.Semantic proximity Read

Massively Parallel Profiling of RNA-targeting CRISPR-Cas13dSemantic proximity Read

Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.Semantic proximity Read

Massively parallel profiling of RNA-targeting CRISPR-Cas13dSemantic proximity Read

Frequent, context-dependent effects of human genetic variation on Cas9 activity revealed by population-scale GUIDE-seq-2 and deep combinatorial CHANCE-seq profilingSemantic proximity Read

Comparative characterization of Cas12a2 orthologs identifies high-activity nucleases for programmable cell eliminationSemantic proximity Read

High-throughput evaluation of in vitro CRISPR activities enables optimized large-scale multiplex enrichment of rare variants.Semantic proximity Read

CRISPR-Cas12a REC2 – NUC interactions drive target-strand cleavage and constrain trans cleavageSemantic proximity Read

Cas12e orthologs evolve variable structural elements to facilitate dsDNA cleavageSemantic proximity Read

CasFinder: Flexible algorithm for identifying specific Cas9 targets in genomesSemantic proximity Read

High-fidelity CRISPR-Cas9 nucleases with no detectable genome-wide off-target effects.Semantic proximity Read

Engineered PAM-flexible FnCas9 variants for robust and specific genome editing and diagnosticsSemantic proximity Read