Article
A universal and sensitive gene mutation detection method based on CRISPR-Cas12a.
Analytica chimica acta - 15 Mar 2023
Wang Huajing, Liu Ruijie, Dong Kejun, Zhang Lei, Zhang Jingxi, Zhang Xiaoping, Zhang Jiarui, Xiao Xianjin, Zhang Wei, Wang Xinyu
Abstract excerpt
Single nucleotide mutations are highly related to the occurrence and development of cancer. The development of simple single nucleotide mutation detection methods with high sensitivity and specificity has great clinical significance for the prevention, diagnosis, treatment and prognosis evaluation of cancer. In recent years, CRISPR/Cas12a has been developed as a highly sensitive, simple and fast tool for nucleic...
Topics
- CRISPR-Cas Systems
- Clinical Relevance
- Mutation
- Nucleotides
- Polymerase Chain Reaction
- Nucleic Acid Amplification Techniques