Article
Mitochondrial base editor induces substantial nuclear off-target mutations.
Nature - 1 Jun 2022
Lei Zhixin, Meng Haowei, Liu Lulu, Zhao Huanan, Rao Xichen, Yan Yongchang, Wu Hao, Liu Min, He Aibin, Yi Chengqi
Abstract excerpt
DddA-derived cytosine base editors (DdCBEs)-which are fusions of split DddA halves and transcription activator-like effector (TALE) array proteins from bacteria-enable targeted C•G-to-T•A conversions in mitochondrial DNA1. However, their genome-wide specificity is poorly understood. Here we show that the mitochondrial base editor induces extensive off-target editing in the nuclear genome. Genome-wide, unbiased...
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