Article

Sensitive, direct detection of non-coding off-target base editor unwinding and editing in primary cells

2025-09-25

Abstract excerpt

Base editors create precise nucleotide changes in DNA, but their off-target activity remains challenging to quantify. Here, we develop and deploy a direct, in cellulo sequencing assay that simultaneously measures both Cas9-mediated unwinding and deaminase editing of genomic DNA (beCasKAS). Our strategy nominates >460-fold more potential off-target sites than other methods by enriching for Cas9-dependent R-loops i...

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Identifiers and source

Literature Corpus work: 5ecbaf26-004f-58c4-b944-06cc7719d871
DOI: 10.1101/2025.09.25.678665

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