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Article

Efficient DNA interrogation of SpCas9 governed by its electrostatic interaction with DNA beyond the PAM and protospacer.

Nucleic acids research - 2 Dec 2021

Zhang Qian, Chen Ziting, Wang Fangzhu, Zhang Siqi, Chen Hongyu, Gu Xueying, Wen Fengcai, Jin Jiachuan, Zhang Xia, Huang Xingxu, Shen Bin, Sun Bo

Abstract excerpt

Streptococcus pyogenes Cas9 (SpCas9), a programmable RNA-guided DNA endonuclease, has been widely repurposed for biological and medical applications. Critical interactions between SpCas9 and DNA confer the high specificity of the enzyme in genome engineering. Here, we unveil that an essential SpCas9-DNA interaction located beyond the protospacer adjacent motif (PAM) is realized through electrostatic forces...

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Identifiers

PMID: 34850124
DOI: 10.1093/nar/gkab1139
PMCID: PMC8643646

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