Article
CRISPR/Cas9-mediated base-editing enables a chain reaction through sequential repair of sgRNA scaffold mutations.
Scientific reports - 13 Dec 2021
Fukushima Tsuyoshi, Tanaka Yosuke, Adachi Keito, Masuyama Nanami, Tsuchiya Akiho, Asada Shuhei, Ishiguro Soh, Mori Hideto, Seki Motoaki, Yachie Nozomu, Goyama Susumu, Kitamura Toshio
Abstract excerpt
Cell behavior is controlled by complex gene regulatory networks. Although studies have uncovered diverse roles of individual genes, it has been challenging to record or control sequential genetic events in living cells. In this study, we designed two cellular chain reaction systems that enable sequential sgRNA activation in mammalian cells using a nickase Cas9 tethering of a cytosine nucleotide deaminase...
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