Utilization of nicking properties of CRISPR-Cas12a effector for genome editing

Abstract excerpt

The CRISPR-Cas nickase system for genome editing has attracted considerable attention owing to its safety, efficiency, and versatility. Although alternative effectors to Cas9 have the potential to expand the scope of genome editing, their application has not been optimized. Herein, we used an enhanced CRISPR-Cas12a nickase system to induce mutations by targeting genes in a human-derived cell line. The newly develo...

Topics

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• Cell Line
• CRISPR and Genetic Engineering
• CRISPR-Associated Protein 9
• CRISPR-Cas Systems
• Deoxyribonuclease I
• DNA Breaks, Double-Stranded
• Gene Editing
• Mutation

Identifiers and source

Literature Corpus work

a551c2a5-31a7-5655-9cab-fe1a12ea9afa

DOI

10.21203/rs.3.rs-3232775/v1