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Article

Shortening the sgRNA-DNA interface enables SpCas9 and eSpCas9(1.1) to nick the target DNA strand.

Science China. Life sciences - 1 Nov 2020

Fan Rong, Chai Zhuangzhuang, Xing Sinian, Chen Kunling, Qiu Fengti, Chai Tuanyao, Qiu Jin-Long, Zhang Zhengbin, Zhang Huawei, Gao Caixia

Abstract excerpt

The length of the sgRNA-DNA complementary sequence is a key factor influencing the cleavage activity of Streptococcus pyogenes Cas9 (SpCas9) and its variants. The detailed mechanism remains unknown. Here, based on in vitro cleavage assays and base editing analysis, we demonstrate that reducing the length of this complementary region can confer nickase activity on SpCas9 and eSpCas9(1.1). We also show that these...

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PMID: 32592086
DOI: 10.1007/s11427-020-1722-0

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026.

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