Article
Direct characterization of cis-regulatory elements and functional dissection of complex genetic associations using HCR-FlowFISH.
Nature genetics - 1 Aug 2021
Reilly Steven K, Gosai Sager J, Gutierrez Alan, Mackay-Smith Ava, Ulirsch Jacob C, Kanai Masahiro, Mouri Kousuke, Berenzy Daniel, Kales Susan, Butler Gina M, Gladden-Young Adrianne, Bhuiyan Redwan M, Stitzel Michael L, Finucane Hilary K, Sabeti Pardis C, Tewhey Ryan
Abstract excerpt
Effective interpretation of genome function and genetic variation requires a shift from epigenetic mapping of cis-regulatory elements (CREs) to characterization of endogenous function. We developed hybridization chain reaction fluorescence in situ hybridization coupled with flow cytometry (HCR-FlowFISH), a broadly applicable approach to characterize CRISPR-perturbed CREs via accurate quantification of native...
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