Article
Human blood PIG-A mutation and micronucleated reticulocyte flow cytometric assays: Method optimization and evaluation of intra- and inter-subject variation.
Environmental and molecular mutagenesis - 1 Oct 2020
Torous Dorothea K, Avlasevich Svetlana L, Khattab Mona G, Baig Ayesha, Saubermann Lawrence J, Chen Yuhchyau, Bemis Jeffrey C, Lovell David P, Walker Vernon E, MacGregor James T, Dertinger Stephen D
Abstract excerpt
We previously described flow cytometry-based methods for scoring the incidence of micronucleated reticulocytes (MN-RET) and PIG-A mutant phenotype reticulocytes (MUT RET) in rodent and human blood samples. The current report describes important methodological improvements for human blood analyses, including immunomagnetic enrichment of CD71-positive reticulocytes prior to MN-RET scoring, and procedures for...
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