Article
The PIGRET assay, a method for measuring Pig-a gene mutation in reticulocytes, is reliable as a short-term in vivo genotoxicity test: Summary of the MMS/JEMS-collaborative study across 16 laboratories using 24 chemicals.
Mutation research. Genetic toxicology and environmental mutagenesis - 15 Nov 2016
Kimoto Takafumi, Horibata Katsuyoshi, Miura Daishiro, Chikura Satsuki, Okada Yuki, Ukai Akiko, Itoh Satoru, Nakayama Shiho, Sanada Hisakazu, Koyama Naomi, Muto Shigeharu, Uno Yoshifumi, Yamamoto Mika, Suzuki Yuta, Fukuda Takayuki, Goto Ken, Wada Kunio, Kyoya Takahiro, Shigano Miyuki, Takasawa Hironao, Hamada Shuichi, Adachi Hideki, Uematsu Yasuaki, Tsutsumi Eri, Hori Hisako, Kikuzuki Ryuta, Ogiwara Yosuke, Yoshida Ikuma, Maeda Akihisa, Narumi Kazunori, Fujiishi Yohei, Morita Takeshi, Yamada Masami, Honma Masamitsu
Abstract excerpt
The in vivo mutation assay using the X-linked phosphatidylinositol glycan class A gene (Pig-a in rodents, PIG-A in humans) is a promising tool for evaluating the mutagenicity of chemicals. Approaches for measuring Pig-a mutant cells have focused on peripheral red blood cells (RBCs) and reticulocytes (RETs) from rodents. The recently developed PIGRET assay is capable of screening >1×106 RETs for Pig-a mutants by...
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