Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Human erythrocyte PIG-A assay: an easily monitored index of gene mutation requiring low volume blood samples.

Environmental and molecular mutagenesis - 1 May 2015

Dertinger Stephen D, Avlasevich Svetlana L, Bemis Jeffrey C, Chen Yuhchyau, MacGregor James T

Abstract excerpt

This laboratory has previously described a method for scoring the incidence of rodent blood Pig-a mutant phenotype erythrocytes using immunomagnetic separation in conjunction with flow cytometric analysis (In Vivo MutaFlow®). The current work extends this approach to human blood. The frequencies of CD59- and CD55-negative reticulocytes (RET(CD59-/CD55-)) and erythrocytes (RBC(CD59-/CD55-)) serve as phenotypic...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Blood Specimen Collection
Share to CD55 Antigens

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to CD59 Antigens

Share to Erythrocytes

Share to Flow Cytometry

Share to Immunomagnetic Separation

Share to Mutation Rate

Share to Reticulocytes

Source

Open in PubMed Open full text

Identifiers

PMID: 25412990
DOI: 10.1002/em.21924
PMCID: PMC4406781

Genes

RET

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Human erythrocyte PIG-A assay: an easily monitored index of gene mutation requiring low volume blood samples.PMID 25412990Read ↗

When pigs fly: immunomagnetic separation facilitates rapid determination of Pig-a mutant frequency by flow cytometric analysis.Semantic proximity Read ↗

Human blood PIG-A mutation and micronucleated reticulocyte flow cytometric assays: Method optimization and evaluation of intra- and inter-subject variation.Semantic proximity Read ↗

Monitoring humans for somatic mutation in the endogenous PIG-a gene using red blood cells.Semantic proximity Read ↗

A population study using the human erythrocyte PIG-A assay.Semantic proximity Read ↗

Recommendations for conducting the rodent erythrocyte Pig-a assay: A report from the HESI GTTC Pig-a Workgroup.Semantic proximity Read ↗

Detection of Pig-a mutant erythrocytes in the peripheral blood of rats and mice.Semantic proximity Read ↗

Detection of Pig-a Mutant Erythrocytes in the Peripheral Blood of Rats and Mice.Semantic proximity Read ↗

The frequency of Pig-a mutant red blood cells in rats exposed in utero to N-ethyl-N-nitrosourea.Semantic proximity Read ↗

In Vivo Pig-a gene mutation assay: Guidance for 3Rs-friendly implementation.Semantic proximity Read ↗

The in vivo Pig-a assay: A report of the International Workshop On Genotoxicity Testing (IWGT) Workgroup.Semantic proximity Read ↗

The Pig-a Gene Mutation Assay in Mice and Human Cells: A Review.Semantic proximity Read ↗

A regenerative erythropoietic response does not increase the frequency of Pig-a mutant reticulocytes and erythrocytes in Sprague-Dawley rats.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.