Article
Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
Nature biotechnology - 1 Jul 2020
Sakata Rina C, Ishiguro Soh, Mori Hideto, Tanaka Mamoru, Tatsuno Kenji, Ueda Hiroki, Yamamoto Shogo, Seki Motoaki, Masuyama Nanami, Nishida Keiji, Nishimasu Hiroshi, Arakawa Kazuharu, Kondo Akihiko, Nureki Osamu, Tomita Masaru, Aburatani Hiroyuki, Yachie Nozomu
Abstract excerpt
We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing...
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