Article
Adenine transversion editors enable precise, efficient A•T-to-C•G base editing in mammalian cells and embryos.
Nature biotechnology - 1 Apr 2024
Chen Liang, Hong Mengjia, Luan Changming, Gao Hongyi, Ru Gaomeng, Guo Xinyuan, Zhang Dujuan, Zhang Shun, Li Changwei, Wu Jun, Randolph Peyton B, Sousa Alexander A, Qu Chao, Zhu Yifan, Guan Yuting, Wang Liren, Liu Mingyao, Feng Bo, Song Gaojie, Liu David R, Li Dali
Abstract excerpt
Base editors have substantial promise in basic research and as therapeutic agents for the correction of pathogenic mutations. The development of adenine transversion editors has posed a particular challenge. Here we report a class of base editors that enable efficient adenine transversion, including precise A•T-to-C•G editing. We found that a fusion of mouse alkyladenine DNA glycosylase (mAAG) with nickase Cas9...
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