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Article

Detection of single nucleotide polymorphism by measuring extension kinetics with T7 exonuclease mediated isothermal amplification.

The Analyst - 18 Dec 2017

Cui Miao, Xiao Xianjin, Zhao Meiping, Zheng Bo

Abstract excerpt

In this work, we measured the primer extension kinetics of the Klenow fragment (exo-) to achieve rapid detection of single nucleotide polymorphism (SNP). Both the matching and the single-base mismatching targets were used as the primer in the kinetic measurements to identify the single nucleotide polymorphism. By coupling with the T7 exonuclease-assisted target cycling process, we decreased the detection limit...

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Source

Identifiers

PMID: 29114669
DOI: 10.1039/c7an00875a

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EGFR

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 5 Sept 2026. Manifest publication-graph-cohort-2026-09-05.1.

Source receipt: pubmed, retrieved 5 Sept 2026.

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