A two-layer assay for single-nucleotide variants utilizing strand displacement and selective digestion.

doi:10.1016/j.bios.2016.03.070

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Article

A two-layer assay for single-nucleotide variants utilizing strand displacement and selective digestion.

Biosensors & bioelectronics - 15 Aug 2016

Yu Yingjie, Wu Tongbo, Johnson-Buck Alexander, Li Lidan, Su Xin

Abstract excerpt

Point mutations have emerged as prominent biomarkers for disease diagnosis, particularly in the case of cancer. Discovering single-nucleotide variants (SNVs) is also of great importance for the identification of single-nucleotide polymorphisms within the population. The competing requirements of thermodynamic stability and specificity in conventional nucleic acid hybridization probes make it challenging to...

Topics

Bacteriophage lambda
Biosensing Techniques
DNA
DNA Mutational Analysis
Exonucleases
Fluorescent Dyes
Humans
Models, Molecular
Nucleotides
Point Mutation
Polymorphism, Single Nucleotide
Spectrometry, Fluorescence

Source

Open in PubMed

Identifiers

PMID: 27100949
DOI: 10.1016/j.bios.2016.03.070

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